Translations Between Differing Liquid Chromatography Formats: Advantages, Principles, and Possible Pitfalls

Translations Between Differing Liquid Chromatography Formats: Advantages, Principles, and Possible Pitfalls

Published online at LCGC North America, August 1, 2014.

The numerous advantages of translating gradient chromatographic methods between the differing formats of liquid chromatography (LC) have been explored and discussed. Although translations in principle obey well-defined chromatographic theories, the authors investigate a number of potential pitfalls that may result in poor translations as exhibited by selectivity differences, changes in efficiency, and hence failure to meet resolution system suitability criteria. The consequences of these pitfalls are examined and the regulatory implications of method translation are explored.

A free translation tool is available to assist the successful translation between HPLC and UHPLC methods. The tool is based on the principles described in this article and permits scaling of gradient times, flow rates, and injection volume as well as accounting for differences in V D/V M ratios between LC systems. You can access this tool here: ACD/Labs LC Method Translator

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