Related presentations, posters, and scientific talks from this event have been posted here for your reference. Please click the associated link to download.
|Complex LC Method Development using Method Development System and LC Simulation Software||K. Zhu, M. Pursch, B. Gu||Download|
|Evaluation of ACD/AutoChrom Software for LC Method Development||G.A. Von Wald and M.T. Vagnini (The Dow Chemical Company)||Download|
|Retention modelling in hydrophilic interaction chromatography (HILIC)||P. Petersson, M. Euerby, K. Kassam, A. Van Wyk, S. Bhal, and I. Oshchepkova||Download|
Complex LC Method Development using Method Development System and LC Simulation Software
Koudi Zhu, Matthias Pursch, Binghe Gu
Wednesday, June 22nd, 3:00 PM–4:15 PM
Yerba Buena Ballroom
Poster Session 4
Retention modelling in hydrophilic interaction chromatography (HILIC)
P. Petersson, M. Euerby, K. Kassam, A. Van Wyk, S. Bhal, and I. Oshchepkova
Thursday, June 23rd, 10:00 AM–11:15 AM
Yerba Buena Ballroom
Poster Session 5
Abstract: View Abstract
HILIC uses hydrophilic stationary phase with reversed phase type eluents. The separation mode of HILIC is more complicated than RPC due to a multi-retention mechanism that may contribute to the overall retention of analytes. The aim of this work was to gain a better understanding of the retention behaviour of: a range of acidic, basic, quaternary ammonium salts; and polar neutral analytes on acidic, basic and neutral stationary phases as a function of a range of HILIC operating parameters such as MeCN content, buffer concentration, pH, and temperature.
A number of both pre-existing and newly developed HILIC retention models were assessed for their ability to predict retention as a function of the HILIC operating parameters using a commercially available retention modelling program (ACD/LC Simulator).
The applicability of these models has been shown in both two dimensional isocratic and one dimensional gradient separations for a wide range of analytes with varying physicochemical properties for each of the three stationary phases investigated. The accuracy of prediction for both retention time and peak width accuracy was observed to be comparable to standard RPC retention modelling, but unfortunately it was discovered that gradient modelling could not be used to predict HILIC isocratic conditions or vice versa. A statistical approach was followed to produce a relative ranking of the importance of HILIC operating parameters on the selectivity and retention of the models. The ranking observed was as follows: the nature of the stationary phase > mobile phase pH (i.e., pH 3-6 mainly effecting the ionization of the analyte) > buffer concentration = organic content > temperature. An understanding of these relative importances should prove valuable to chromatographers in the rational design of robust HILIC method development strategies.