September 23 - 24, 2003, Obernai, France, ACD/Labs European Users' Meeting

RPLC Column Classification and the Development of a Column Selection Tool

Patrik Petersson (AstraZeneca R&D Lund)

Abstract

The identification of LC columns with similar selectivity for replacement or columns with different selectivity for method development is a challenging task considering the large number of columns on the market (approximately 600), the absence of a standardised column characterisation procedure, and the vendor's reluctance to reveal the functionality of their stationary phases. Consequently, there is a large interest in the development of a generic approach for characterising stationary phases.

This paper will describe how 158 RPLC columns have been characterised in terms of their surface coverage, hydrophobic selectivity, shape selectivity, hydrogen bonding capacity, and ion exchange capacity at pH 2.7 and 7.6. It will illustrate how a chemometrical tool, principal component analysis, can be used in order to provide simple graphical comparisons of differences/similarities between columns in the database. In addition, the method by which a column selection tool based on the calculation of distances between columns in the 6 dimensional variable space can be used for the same purpose will be described.

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