April 6, 2016
There are many variants of 1H-13C HSQC experiments that can used for elucidating unknown molecules. In particular, band-selective HSQC data can assist with the data interpretation of correlations with regions exhibiting ambiguous assignments.1
For the following unknown compound, two 1H -13C HSQC spectra were collected. Both datasets (bsgHSQC and gHSQC) were processed with the same set of parameters (F2: window function square sine bell 13.33 Hz at 0.075 s, zerofill to 4k, F1: window function gaussian 12.24 Hz, zerofill to 1k, FLP 256 pts). The band-selective gradient HSQC experiment was collected with less scans and yet the 1JHC could be clearly linked to the respective 13C signals along the F1 axis.
A special thanks goes to John Lowe, Catherine Lyall and Dave Carbery, University of Bath, UK.
bsgHSQC (4 scans) –
Click Download Logic#47_bsgHSQC to expand the image.
gHSQC (8 scans) – Click Download Logic#47_gHSQC to expand the image.