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Qualitative Elucidation via TLC Staining

January 20, 2010

TLC (Thin Layer Chromatography) offers a simple approach to identifying a solvent(s) for separating out a mixture, monitoring a reaction to completion, etc. Furthermore, the TLC plates can be stained to identify the presence or absence of various functional groups such as amines, ketones, etc. This qualitative experiment does offer various drawbacks.

The process of staining typically requires the items shown in the illustration below.

TLCNitrogenStaining_Jan192010

The following websites offer more insight into the process of staining.

http://webpub.allegheny.edu/employee/s/smurphre/research/visualization.pdf

Org Prep Daily: http://orgprepdaily.wordpress.com/2006/09/27/tlc-staining-solutions/

Curly Arrow Blog: http://curlyarrow.blogspot.com/2006/11/lets-talk-about-tlcs-part-1-vanillin.html

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