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Signals can simply disappear on a DEPT-135 experiment

May 20, 2010
by Arvin Moser, Team Manager, Application Scientists, ACD/Labs

There are many advantages in working with a 1H-13C HSQC-DEPT spectrum over a 13C DEPT-135 and a 1H-13C HSQC (see Post 1 & Post 2). In most cases, a 1H-13C HSQC-DEPT is more valuable than either one of those experiments.

The aliphatic region of a 1H-13C HSQC-DEPT is spectrum below. Two coincidental carbon signals are overlapping at 38.51 ppm, one pertaining to a CH while the other a CH2 group. The DEPT-135, attached to the F1 domain, exhibits a weak 13C signal that can easily be misconstrued, for example as an impurity, if not for the extra information from the 2D NMR experiment.

HSQCDEPT_CHoverCH2_May192010

 


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One Reply to “Signals can simply disappear on a DEPT-135 experiment”

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