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What is the difference between Profile and Centroid MS data?

MS data collected off an instrument is presented as either profile or centroid mode. Shown below are two mass spectra illustrating an ion cluster for profile data and a centroid mass spectrum created from the profile data.

In profile mode, a peak is represented by a collection of signals over several scans. The advantage of profile data is it is easier to classify a signal as a true peak from noise off the instrument.

In centroid mode, the signals are displayed as discrete m/z with zero line widths. The advantage of centroid data is the file size is significantly smaller as there is less information describing a signal.

Profile2

4 Replies to “What is the difference between Profile and Centroid MS data?”

  1. Describe please how such signal is generated? Please be so kind to implement info about mass fluctuation. What about resolution of MS?

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