Skip To Content

Comparing band-selective HSQC data with non-selective HSQC data

There are many variants of 1H-13C HSQC experiments that can used for elucidating unknown molecules. In particular, band-selective HSQC data can assist with the data interpretation of correlations with regions exhibiting ambiguous assignments.1

For the following unknown compound, two 1H –13C HSQC spectra were collected. Both datasets (bsgHSQC and gHSQC) were processed with the same set of parameters (F2: window function square sine bell 13.33 Hz at 0.075 s, zerofill to 4k, F1: window function gaussian 12.24 Hz, zerofill to 1k, FLP 256 pts). The band-selective gradient HSQC experiment was collected with less scans and yet the 1JHC could be clearly linked to the respective 13C signals along the F1 axis.

A special thanks goes to John Lowe, Catherine Lyall and Dave Carbery, University of Bath, UK.

  1. Journal of Magnetic Resonance (1999) 139, 454–459.


bsgHSQC (4 scans) –


Click Download Logic#47_bsgHSQC to expand the image.


gHSQC (8 scans) –  Click Download Logic#47_gHSQC to expand the image.