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Determining the Site of Modification … Part 1

In past blogs, I have ascribed the process of peak matching as an integral part of structure elucidation. In this series, peak matching is demonstrated with the use of MS data.

The scheme below shows a parent compound (2-oxo-N,2-diphenylacetamide) with two possible sites for hydrogenation. The potential modified compounds are 2-hydroxy-N,2-diphenylacetamide and 2-hydroxy-1-phenyl-2-(phenylamino)ethanone labeled A and B, respectively.

MS3CompareToAPI_Scheme_Aug252009

The ESI+ full scan MS below belong to the parent compound (top) and the modified compound (bottom). With the given data, can the suspected site of modification be determined?

MS2CompareToAPI_Part1MS1_Aug252009