Process, Analyze and Database Chromatographic Separations with ACD/Chrom Workbook
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ACD/Chrom Workbook

The functionality of ACD/Chrom Workbook has been migrated to ACD/ChemAnalytical Workbook as of ACD/Labs software release 2018.1. Chrom Workbook has been officially retired as a standalone product and will not receive any further updates. Technical support will continue for 3 years from the time of retirement, as per our Software Life Cycle Policy.

For more information on migrating or upgrading, please contact our Sales team:

A Vendor Neutral Environment to Manage HPLC, LC, GC, and CE Separations

ACD/Chrom Workbook is an advanced software tool for processing, analysis, and management of separations data including high performance liquid chromatography (HPLC), liquid chromatography (LC) (with UV, DAD, or PDA detectors), gas chromatography (GC), and capillary electrophoresis (CE).

ACD/Chromatography Applications Database—fully searchable with experimental data for >8100 real-life applications covering HPLC, GC, and CE methods. Instrument and column parameters, solvent and buffer information, chemical structure assignments, and reference sources included.

Supported File Formats

Vendor Data Format Extension Comments
Agilent Technologies 1100 Series LC/MSD Quad and Ion Trap Systems *.ms, *.yep UV, LC-UV and LC-MS
ChemStation Rev. B.02.01, B.03.01, B.04.01, B.04.02, B.04.03, C.01.04 *.D UV, LC-UV and LC-MS
Entire *.D folder should be used
*.ms, *.ch, *.uv
Open Lab C v.1.04 *.D UV, LC-UV and LC-MS
Entire *.D folder should be used
*.ms, *.ch, *.uv
Open Lab Rev. C.01.07, C.01.08, C.01.09 *.D UV, LC-UV and LC-MS
Entire *.D folder should be used
*.ms, *.ch, *.uv
EZChrom *.dat UV traces only.
AB SCIEX Analyst *.wiff LC-UV and LC-MS
Bruker Daltonics and Agilent Technologies Agilent or Bruker LC/MS Ion Trap *.yep LC-UC and LC-MS
Bruker Compass (accurate mass data) *.D LC-MS, LC-UV, UV

Entire *.D folder structure should be used.
Shimadzu Corporation LCMS-IT-TOF *.lcd LC-MS and LC-UV. Requires vendor software on same computer.
LCMSsolution *.qld LC-MS, LC-UV and UV traces

May require vendor software on same computer
Thermo Scientific Xcalibur *.raw LC-MS, LC-UV and UV traces
Chromeleon® 6 UV and LC-UV, via Connect to
Unidata netCDF *.cdf LC-MS, LC-UV and UV traces
Waters Corporation MassLynx *.raw LC-UV and LC-MS
Micromass OpenLynx *.rpt LC-UV and LC-MS
Empower LCUV data *.arw LC-UV
Empower 2 and 3   UV, LC-UV and LC-MS traces, via Connect to.
PerkinElmer® TotalChrom™ *.raw UV chromatograms via Connect to

Support for hyphenated data involving Diode Array Detector (DAD)
"Connect To"

Other Chromatography tools from ACD/Labs

ACD/Labs offers a number of software tools for chromatographic method development, and identification of optimal experiment space:


ACD/Method Selection Suite