HPLC – 54th International Symposium on High Performance Liquid Phase Separations and Related Techniques

Simplify and Streamline Method Transfer  

Authors: Anne Marie Smith, Shahriar Jahanbakht                                                                            

Chromatographic methods are essential for separating and analyzing complex mixtures. Developing robust chromatographic methods is a time-intensive process that requires balancing optimal resolution—especially for critical peak pairs—with efficient runtime. With increased collaboration across facilities and external partners, successful method transfer has become essential in analytical workflows. However, variability in instrument performance caused by small deviations in method conditions can significantly impact separation quality leading to inconsistencies, requiring time-consuming troubleshooting and expert intervention.

To address these challenges, we present modern method development software that automates parameter variation, generates simulated chromatograms, and provides advanced visualization tools such as chromatogram overlays, resolution maps, and integrated data management. These capabilities allow scientists to anticipate risks, refine methods before transfer, and identify conditions that may impact performance. By simulating chromatographic variations, analysts can assess sensitivity of method parameter fluctuations and pinpoint critical parameters to ensure robustness and quality.

With these insights, scientists can make data-driven adjustments, reducing troubleshooting time and ensuring seamless method transfer across instruments and labs. By leveraging software-driven automation and visualization, teams can optimize time and material resources, minimize variability, and enhance collaboration across analytical workflows.