Skip To Content

ACD/Labs Blog

Chromatography aims to separate an analyte mixture into sharp, symmetrical, and well-resolved peaks for quantitative and qualitative analysis. To do this, it is crucial to understand ionization and the ionic form(s) of analytes at a given pH. The use of predictive software tools allows quick selection of optimal conditions and helps build robustness into methods from the start.

Mass spectrometry is a powerful analytical tool that enables scientists to identify and quantify molecules with remarkable precision. At the heart of MS lies the ionization process. Understanding the different ionization methods in mass spectrometry enables selection of the most appropriate technique to accurately analyze your data.

Mass spectrometry (MS) is a valuable tool that can give you vast amounts of MS data to help you identify and quantify components. MS has been used to discover, determine, and quantify sample compounds in the proteomics, metabolomics, imaging, and glycomics applications across the food, pharmaceutical, and environmental industries. Combining MS with appropriate software and computer technology allows the possibility for more extensive applications. But first, we must understand the basics.

The highly coveted Gaussian peak, a sharp symmetrical shape on a flat baseline, is the ideal peak shape in chromatography. Why is it so highly desired? Naturally, we tend to be drawn to the beauty of symmetry, but more importantly, good peak shape is important for better resolution (Rs) and increased accuracy in quantitation.