September 14, 2009
by Arvin Moser, Team Manager, Application Scientists, ACD/Labs
Several NMR experiments offer tools to help determine the stereochemistry of a structure. Some typical experiments are 1D NOE (Nuclear Overhauser Effect), 2D NOESY (NOE Spectroscopy) and ROESY (Rotating-frame Overhauser Effect Spectroscopy). These experiments will produce signals for nuclei that are close to each other through space independent of the number of bonds separating the nuclei.
A simplified 1H-1H NOESY spectrum is shown below. The spectrum shows 2 correlations at (4.29,1.28) and (4.29,3.13) ppm. There is no correlation to the proton signal at 2.68 ppm.
![NOESY_Part1Spec_Sept142009 NOESY_Part1Spec_Sept142009](https://www.acdlabs.com/wp-content/uploads/2022/06/img_62a9fe6ec9e36.gif)
Based on NOESY data, there are 2 possible conformations. The enantiomers (partially drawn) are shown below.
![NOESY_Part1Str_Sept142009 NOESY_Part1Str_Sept142009](https://www.acdlabs.com/wp-content/uploads/2022/06/img_62a9fe72bc0ab.gif)